Biosynthesis of D-aspartic acid by Streptococcus faecalis.

نویسندگان

  • M Rahmanian
  • G R Waller
  • W G Smith
چکیده

In biotin deficiency, aspartic acid becomes a limiting factor in the growth of Streptococcus faecalis ATCC 9190. Suboptimal concentrations of L-aspartate in biotin-deficient medium produce partial growth followed by lysis, which is typical of cell wall lysis due to depletion of an amino acid essential for cell wall biosynthesis. D-Aspartate, which is utilized by the cells with considerable less efficiency than the L isomer, can prevent depletion lysis when present at high concentrations. Irrespective of aspartate concentration, a minimal concentration of biotin is so indispensable for normal growth that at lower biotin concentrations partial growth occurs followed by cell lysis. L-AspartateJ4C included in the growth medium is incorporated into D-aspartate isolated from the cell wall. Dilution with endogenously synthesized amino acid is a function of aspartate and biotin concentrations. Wall and protein aspartic acids were isolated from cells grown with DL-aSpSrtate-l-r% or with DL-aspartate-4J4C and selectively decarboxylated with chloramine-T in alkali to locate the position of 14C in the molecule. Lack of randomization of 14C between the orand /3-carboxyl groups indicated the absence of a symmetrical intermediate with enzymically indistinguishable carboxyl groups (fumarate) in the pathway between Land D-aspartic acid. Cell wall D-aspartate isolated from cells grown on L-aspartateJ6N contained nearly the same 16N content as the r,-aspartate in the medium, indicating that D-aspartate is formed from L-aspartate via racemization.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Effect of carbon dioxide on the aspartic acid requirement for proteinase biosynthesis by Streptococcus faecalis var. liquefaciens.

Non-proliferating cells of Streptococcus faecalis var. liquefaciens required aspartic acid for proteinase biosynthesis in the absence of CO(2) but not in the presence of CO(2).

متن کامل

Biosynthesis of D-Aspartic Acid by Streptococcus faecalis*

In biotin deficiency, aspartic acid becomes a limiting factor in the growth of Streptococcus faecalis ATCC 9190. Suboptimal concentrations of L-aspartate in biotin-deficient medium produce partial growth followed by lysis, which is typical of cell wall lysis due to depletion of an amino acid essential for cell wall biosynthesis. D-Aspartate, which is utilized by the cells with considerable less...

متن کامل

Transport of aminophosphonic acids in Lactobacillus plantarum and Streptococcus faecalis.

Aminophosphonic acids analogous to glutamic acid, aspartic acid, alanine, and valine were actively accumulated by Lactobacillus plantarum. Uptake was dependent on the availability of glucose and, in all cases, the estimated intracellular concentrations substantially exceeded extracellular levels. During uptake, there was little metabolism of tritiated 2-amino-3-phosphonopropionic acid (APP), th...

متن کامل

Protoplast membrane of Streptococcus faecalis.

Shockman, Gerald D. (Temple University School of Medicine, Philadelphia, Pa.), Joseph J. Kolb, Bohdan Bakay, Margaret J. Conover, and Gerrit Toennies. Protoplast membrane of Streptococcus faecalis. J. Bacteriol. 85:168-176. 1963.-The membrane fraction of Streptococcus faecalis (ATCC 9790) was isolated and purified, by a variety of procedures, from cultures that were grown under closely controll...

متن کامل

Dependence of protease secretion by Streptococcus faecalis var. liquefaciens on arginine and its possible relation to site of synthesis.

Washed cells of Streptococcus faecalis var. liquefaciens, when harvested from media that supported protease biosynthesis, continued to release this extracellular enzyme in phosphate buffer. The addition of ethylenediaminetetraacetic acid (EDTA) halted the secretion. If zinc ions were added to the EDTA-treated cells before 45 to 60 min had elapsed, a fraction of the anticipated enzyme activity w...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • The Journal of biological chemistry

دوره 246 3  شماره 

صفحات  -

تاریخ انتشار 1971